L-02-CAS9 cell line

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L-02-CAS9 (Hela-contaminated cells, not for sales) Order

Catalog#

YC-D004-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium(L-02)

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

L-02-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

80% RPMI-1640+20% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the L-02 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a L-02-CAS9 cell line that can maintain long-term, stable and high expression.

Cutting Efficiency Validation

L-02-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the L-02-CAS9 stable cell pool which is electroporated by HIF1A gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:L-02			STR Info (Cell bank) Cell Line:L-02
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	13.3	13.3		13.3	13.3
D7S820	12	12		12	12
D16S539	9	10		9	10
VWA	16	16		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	12	12		12	12
CSF1PO	10	10		10	10
D12S391	20	20
FGA	18	21
D2S1338	17	17
D21S11	27	28
D18S51	16	16
D8S1179	12	12
D3S1358	15	18
D6S1043	18	18
PENTAE	7	17
D19S433	13	13
PENTAD	8	15
D1S1656	12	15

* Hela-contaminated cells, not for sales

Reviewed by ICLAC (ref: 190616). L-02 was reported to be established from normal liver cells but its STR profile corresponds to HeLa. Samples tested in this study were directly deposited in the cell line repository that performed the investigation. L-02 has a derivative: HL-7702BaPT. No STR profiling information was available for the derivative at the time of review.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

L-02-CAS9

Catalog#

YC-D004-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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L-02-CAS9 cell line

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